Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition
University of Copenhagen · Novo Nordisk Foundation · +6 more institutions
Abstract
Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes in a fast and reproducible manner. Here we present the narrow-window data-independent acquisition (nDIA) strategy consisting of high-resolution MS1 scans with parallel tandem MS (MS/MS) scans of ~200 Hz using 2-Th isolation windows, dissolving the differences between data-dependent and -independent methods. This is achieved by pairing a quadrupole Orbitrap mass spectrometer with the asymmetric track lossless (Astral) analyzer which provides >200-Hz MS/MS scanning speed, high resolving power and sensitivity, and low-ppm mass accuracy. The nDIA strategy enables profiling of >100 full yeast proteomes per day, or 48 human…
Citation impact
- FWCI
- 68.54
- Percentile
- 100%
- References
- 71
Authors
22- UHUlises H. GuzmánCorresponding
University of Copenhagen, Novo Nordisk Foundation
- AMAna Martínez‐Val
University of Copenhagen, Novo Nordisk Foundation
- ZYZilu Ye
University of Copenhagen, Chinese Academy of Medical Sciences & Peking Union Medical College, Novo Nordisk Foundation, Suzhou Institute of Systems Medicine
- EDEugen Damoc
Thermo Fisher Scientific (Germany)
- TNTabiwang N. Arrey
Thermo Fisher Scientific (Germany)
Topics & keywords
- Proteome
- Mass spectrometry
- Quantitative proteomics
- Chemistry
- Tandem mass spectrometry
- Proteomics
- Tandem mass tag
- Orbitrap