articleCell stem cellFeb 1, 2024HYBRID OA

3D bioprinting of human neural tissues with functional connectivity

YYYuanwei YanXLXueyan LiYGYu GaoSMSakthikumar MathivananLKLinghai Kong

University of Wisconsin–Madison · Research Network (United States) · +2 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Probing how human neural networks operate is hindered by the lack of reliable human neural tissues amenable to the dynamic functional assessment of neural circuits. We developed a 3D bioprinting platform to assemble tissues with defined human neural cell types in a desired dimension using a commercial bioprinter. The printed neuronal progenitors differentiate into neurons and form functional neural circuits within and between tissue layers with specificity within weeks, evidenced by the cortical-to-striatal projection, spontaneous synaptic currents, and synaptic response to neuronal excitation. Printed astrocyte progenitors develop into mature astrocytes with elaborated processes and form functional…

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115
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FWCI
19.32
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100%
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70
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Authors

11

Topics & keywords

Topics
Keywords
  • Neuroscience
  • Biological neural network
  • Biology
  • Neural stem cell
  • Astrocyte
  • Glutamate receptor
  • Calcium imaging
  • Nerve net
UN Sustainable Development Goals
  • Good health and well-being
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