G-quadruplex DNA structure is a positive regulator of MYC transcription
University of Cambridge · Cancer Research UK
Abstract
DNA structure can regulate genome function. Four-stranded DNA G-quadruplex (G4) structures have been implicated in transcriptional regulation; however, previous studies have not directly addressed the role of an individual G4 within its endogenous cellular context. Using CRISPR to genetically abrogate endogenous G4 structure folding, we directly interrogate the G4 found within the upstream regulatory region of the critical human MYC oncogene. G4 loss leads to suppression of MYC transcription from the P1 promoter that is mediated by the deposition of a de novo nucleosome alongside alterations in RNA polymerase recruitment. We also show that replacement of the endogenous MYC G4 with a different G4 structure from…
Citation impact
- FWCI
- 27.43
- Percentile
- 100%
- References
- 61
Authors
11- IEIsabel Esain-GarciaCorresponding
University of Cambridge, Cancer Research UK
- AKAngie KirchnerCorresponding
University of Cambridge, Cancer Research UK
- LMLarry Melidis
University of Cambridge, Cancer Research UK
- RDRafael de Cesaris Araujo Tavares
University of Cambridge, Cancer Research UK
- SDSomdutta Dhir
University of Cambridge, Cancer Research UK
Topics & keywords
- Biology
- Chromatin
- Transcription (linguistics)
- Nucleosome
- DNA
- Promoter
- Genetics
- Transcriptional regulation