Bile salt hydrolase catalyses formation of amine-conjugated bile acids

; however, the bacterial gene(s) responsible for their production remain unknown. Herein, we report that bile salt hydrolase (BSH) possesses dual functions in bile acid metabolism. Specifically, we identified a previously unknown role for BSH as an amine N-acyltransferase that conjugates amines to bile acids, thus forming bacterial bile acid amidates (BBAAs). To characterize this amine N-acyltransferase BSH activity, we used pharmacological inhibition of BSH, heterologous expression of bsh and mutants in Escherichia coli and bsh knockout and complementation in Bacteroides fragilis to demonstrate that BSH generates BBAAs. We further show in a human infant cohort that BBAA production is positively correlated…

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  American Heart Association
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  Pennsylvania Department of Health

  Award: UL1TR001878
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  Children's Hospital of Philadelphia

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• UO
  University of Pennsylvania

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  Pennsylvania State University
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  Crohn's and Colitis Foundation

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  American Beverage Foundation for a Healthy America
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  National Institutes of Health

  Awards: T32GM102057, R01DK095201, UL1TR001878, R35GM138369, T32DK120509, R01CA245546, P50CA130810, S10OD021750, DK034933, 1U24DK133658-01, P30CA046592, R01CA148828, P30DK050306
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  University of Michigan Comprehensive Cancer Center

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  National Cancer Institute

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  National Institute of Diabetes and Digestive and Kidney Diseases

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  National Center for Research Resources

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