reviewChemical ReviewsFeb 14, 2024Closed access

Design and Application of Fluorescent Probes to Detect Cellular Physical Microenvironments

Westlake University · Dalian Institute of Chemical Physics · +2 more institutions

PubMed
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Abstract

The microenvironment is indispensable for functionality of various biomacromolecules, subcellular compartments, living cells, and organisms. In particular, physical properties within the biological microenvironment could exert profound effects on both the cellular physiology and pathology, with parameters including the polarity, viscosity, pH, and other relevant factors. There is a significant demand to directly visualize and quantitatively measure the fluctuation in the cellular microenvironment with spatiotemporal resolution. To satisfy this need, analytical methods based on fluorescence probes offer great opportunities due to the facile, sensitive, and dynamic detection that these molecules could enable in…

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