Computational redesign of a hydrolase for nearly complete PET depolymerization at industrially relevant high-solids loading
Chinese Academy of Sciences · Institute of Automation · +4 more institutions
Abstract
Abstract Biotechnological plastic recycling has emerged as a suitable option for addressing the pollution crisis. A major breakthrough in the biodegradation of poly(ethylene terephthalate) (PET) is achieved by using a LCC variant, which permits 90% conversion at an industrial level. Despite the achievements, its applications have been hampered by the remaining 10% of nonbiodegradable PET. Herein, we address current challenges by employing a computational strategy to engineer a hydrolase from the bacterium HR29. The redesigned variant, TurboPETase, outperforms other well-known PET hydrolases. Nearly complete depolymerization is accomplished in 8 h at a solids loading of 200 g kg −1 . Kinetic and structural…
Citation impact
- FWCI
- 18.83
- Percentile
- 100%
- References
- 66
Authors
8- YCYinglu CuiCorresponding
Chinese Academy of Sciences, Institute of Automation, Institute of Microbiology
- YCYanchun Chen
Chinese Academy of Sciences, Institute of Microbiology
- JSJinyuan Sun
Chinese Academy of Sciences, Institute of Microbiology, University of Chinese Academy of Sciences
- TZTong Zhu
Chinese Academy of Sciences, Institute of Microbiology
- HPHua Pang
Chinese Academy of Sciences, Institute of Microbiology
Topics & keywords
- Depolymerization
- Polyester
- Hydrolase
- Biodegradation
- Biochemical engineering
- Polymer
- Materials science
- Computer science
Funding
- NNNational Natural Science Foundation of ChinaAwards: ZDBS-LY-SM014, 2021YFC2103600, 32225002, 32170033, 31822002, KFJ-BRP-009
- CAChinese Academy of SciencesAwards: CAS-WX2021SF-0111, KFJ-BRP-009, KFJ-BRP-017-58, ZDBS-LY-SM014
- YIYouth Innovation Promotion Association of the Chinese Academy of SciencesAward: 2022086
- YIYouth Innovation Promotion Association