Generation of complex bone marrow organoids from human induced pluripotent stem cells

The human bone marrow (BM) niche sustains hematopoiesis throughout life. We present a method for generating complex BM-like organoids (BMOs) from human induced pluripotent stem cells (iPSCs). BMOs consist of key cell types that self-organize into spatially defined three-dimensional structures mimicking cellular, structural and molecular characteristics of the hematopoietic microenvironment. Functional properties of BMOs include the presence of an in vivo-like vascular network, the presence of multipotent mesenchymal stem/progenitor cells, the support of neutrophil differentiation and responsiveness to inflammatory stimuli. Single-cell RNA sequencing revealed a heterocellular composition including the presence…

• LM
  Leona M. and Harry B. Helmsley Charitable Trust
• DZ
  Deutsches Zentrum für Infektionsforschung
• EF
  European Federation of Pharmaceutical Industries and Associations
• EC
  European Commission

  Award: 101005026
• DF
  Deutsche Forschungsgemeinschaft

  Awards: INST 86/1909-1, 458247426, CRC1123, SFB914, TRR332, 1FUGG
• FL
  Fondation Leducq

  Award: 21CVD03
• ÖA
  Österreichischen Akademie der Wissenschaften

  Award: 101005026
• BF
  Bundesministerium für Bildung und Forschung

  Award: 01KX2324
• EK
  Else Kröner-Fresenius-Stiftung
• UW
  Universität Wien
• LM
  Ludwig-Maximilians-Universität München
• MU
  Medizinische Universität Wien
• BF
  Bundesministerium für Bildung, Wissenschaft und Forschung
• HS
  Hector Stiftung