Spatially organized cellular communities form the developing human heart

. However, how diverse cardiac cell types spatially coordinate to create the complex morphological structures that are crucial for heart function remains unclear. Here we integrated single-cell RNA-sequencing with high-resolution multiplexed error-robust fluorescence in situ hybridization to resolve the identity of the cardiac cell types that develop the human heart. This approach also provided a spatial mapping of individual cells that enables illumination of their organization into cellular communities that form distinct cardiac structures. We discovered that many of these cardiac cell types further specified into subpopulations exclusive to specific communities, which support their specialization according…

• NS
  National Science Foundation

  Award: 2135720
• CI
  California Institute for Regenerative Medicine

  Awards: GC1R-06673, GC1R-06673-B
• NI
  National Institutes of Health
• UO
  University of California, San Diego