Assessing GPT-4 for cell type annotation in single-cell RNA-seq analysis

Here we demonstrate that the large language model GPT-4 can accurately annotate cell types using marker gene information in single-cell RNA sequencing analysis. When evaluated across hundreds of tissue and cell types, GPT-4 generates cell type annotations exhibiting strong concordance with manual annotations. This capability can considerably reduce the effort and expertise required for cell type annotation. Additionally, we have developed an R software package GPTCelltype for GPT-4's automated cell type annotation.

• NI
  National Institutes of Health

  Award: U54AG075936
• SO
  School of Medicine, Duke University
• NI
  National Institute of General Medical Sciences