articleCellMar 1, 2024HYBRID OA

Spatial proteomics in neurons at single-protein resolution

EMEduard M. UnterauerSSSayedali Shetab BoushehriKJKristina JevdokimenkoLALuciano A. MasulloMGMahipal Ganji

Center for NanoScience · Max Planck Institute of Biochemistry · +8 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

To understand biological processes, it is necessary to reveal the molecular heterogeneity of cells by gaining access to the location and interaction of all biomolecules. Significant advances were achieved by super-resolution microscopy, but such methods are still far from reaching the multiplexing capacity of proteomics. Here, we introduce secondary label-based unlimited multiplexed DNA-PAINT (SUM-PAINT), a high-throughput imaging method that is capable of achieving virtually unlimited multiplexing at better than 15 nm resolution. Using SUM-PAINT, we generated 30-plex single-molecule resolved datasets in neurons and adapted omics-inspired analysis for data exploration. This allowed us to reveal the complexity…

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138
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Authors

14

Topics & keywords

Topics
Keywords
  • Proteomics
  • Biology
  • Computational biology
  • Workflow
  • Multiplexing
  • Resolution (logic)
  • Computer science
  • Artificial intelligence
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