Spatial transcriptomics reveals discrete tumour microenvironments and autocrine loops within ovarian cancer subclones
The University of Western Australia · Harry Perkins Institute of Medical Research · +7 more institutions
Abstract
High-grade serous ovarian carcinoma (HGSOC) is genetically unstable and characterised by the presence of subclones with distinct genotypes. Intratumoural heterogeneity is linked to recurrence, chemotherapy resistance, and poor prognosis. Here, we use spatial transcriptomics to identify HGSOC subclones and study their association with infiltrating cell populations. Visium spatial transcriptomics reveals multiple tumour subclones with different copy number alterations present within individual tumour sections. These subclones differentially express various ligands and receptors and are predicted to differentially associate with different stromal and immune cell populations. In one sample, CosMx single molecule…
Citation impact
- FWCI
- 27.41
- Percentile
- 100%
- References
- 79
Authors
22- EDElena DenisenkoCorresponding
The University of Western Australia, Harry Perkins Institute of Medical Research, Queen Elizabeth II Medical Centre
- LDLeanne de Kock
The University of Western Australia, Children's Hospital of Eastern Ontario, Harry Perkins Institute of Medical Research, Queen Elizabeth II Medical Centre
- ATAdeline Tan
- ABAaron B. Beasley
Edith Cowan University
- MBMaria Beilin
St John of God Subiaco Hospital
Topics & keywords
- Stromal cell
- Biology
- Autocrine signalling
- Cancer research
- Transcriptome
- Immune system
- Ovarian carcinoma
- Tumor microenvironment
- No poverty