Improving prime editing with an endogenous small RNA-binding protein

Yan, Jun; Oyler, Paul; Ravisankar, Purnima; Ward, Carl C.; Levesque, Sébastien; Jing, Yangwode; Simpson, Danny; Zhao, Anqi; Li, Hui; Yan, Weihao; Goudy, Laine; Schmidt, Ralf; Solley, Sabrina C.; Gilbert, Luke A.; Chan, Michelle M.; Bauer, Daniel E.; Marson, Alexander; Parsons, Lance; Adamson, Britt

doi:10.1038/s41586-024-07259-6

articleNatureApr 3, 2024HYBRID OA

Improving prime editing with an endogenous small RNA-binding protein

JYJun Yan POPaul Oyler PRPurnima Ravisankar CCCarl C. Ward SLSébastien Levesque

Princeton University · Cornell University · +14 more institutions

PubMed

Indexed incrossrefpubmed

Abstract

Abstract Prime editing enables the precise modification of genomes through reverse transcription of template sequences appended to the 3′ ends of CRISPR–Cas guide RNAs 1 . To identify cellular determinants of prime editing, we developed scalable prime editing reporters and performed genome-scale CRISPR-interference screens. From these screens, a single factor emerged as the strongest mediator of prime editing: the small RNA-binding exonuclease protection factor La. Further investigation revealed that La promotes prime editing across approaches (PE2, PE3, PE4 and PE5), edit types (substitutions, insertions and deletions), endogenous loci and cell types but has no consistent effect on genome-editing approaches…

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210

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FWCI: 43.94
Percentile: 100%
References: 64

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Topics & keywords

Topics

Keywords

RNA editing
Prime (order theory)
Computational biology
RNA
Genome editing
Biology
CRISPR
Genetics

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