Tracing the origin of alveolar stem cells in lung repair and regeneration
Center for Excellence in Molecular Cell Science · University of Chinese Academy of Sciences · +1 more institution
Abstract
Alveolar type 2 (AT2) cells are stem cells of the alveolar epithelia. Previous genetic lineage tracing studies reported multiple cellular origins for AT2 cells after injury. However, conventional lineage tracing based on Cre-loxP has the limitation of non-specific labeling. Here, we introduced a dual recombinase-mediated intersectional genetic lineage tracing approach, enabling precise investigation of AT2 cellular origins during lung homeostasis, injury, and repair. We found AT1 cells, being terminally differentiated, did not contribute to AT2 cells after lung injury and repair. Distinctive yet simultaneous labeling of club cells, bronchioalveolar stem cells (BASCs), and existing AT2 cells revealed the exact…
Citation impact
- FWCI
- 41.62
- Percentile
- 100%
- References
- 44
Authors
12- KLKuo Liu
Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences
- XMXinfeng Meng
ShanghaiTech University
- ZLZixin Liu
Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences
- MTMuxue Tang
Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences
- ZLZan Lv
Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences
Topics & keywords
- Biology
- Stem cell
- Cell biology
- Regeneration (biology)
- Lung
- Cell type
- Lineage (genetic)
- Recombinase
- Good health and well-being