Composition and metabolism of microbial communities in soil pores

Abstract Delineation of microbial habitats within the soil matrix and characterization of their environments and metabolic processes are crucial to understand soil functioning, yet their experimental identification remains persistently limited. We combined single- and triple-energy X-ray computed microtomography with pore specific allocation of 13 C labeled glucose and subsequent stable isotope probing to demonstrate how long-term disparities in vegetation history modify spatial distribution patterns of soil pore and particulate organic matter drivers of microbial habitats, and to probe bacterial communities populating such habitats. Here we show striking differences between large (30-150 µm Ø) and small (4-10…

• NS
  National Science Foundation

  Awards: 1904267, DE-SC0018409, 1027253, DEB 1027253, DE-AC02-06CH11357
• UD
  U.S. Department of Energy

  Awards: COVID-19, AC02-06CH11357, SC0018409, DE-SC0018409, DE-AC02, 06CH11357, DE-AC02-06CH11357, DE-AC02-
• UD
  U.S. Department of Agriculture

  Awards: DE-AC02-06CH11357, 67019
• MS
  Michigan State University

  Award: DE-AC02-06CH11357
• DF
  Deutsche Forschungsgemeinschaft

  Awards: SPP2322, DE-AC02-06CH11357, 465122443
• NI
  National Institute of Food and Agriculture

  Award: DE-SC0018409
• OO
  Office of Science

  Awards: DE-AC02-06CH11357, DE-AC02, DE-SC0018409, 06CH11357, AC02-06CH11357
• CO
  College of Engineering, Michigan State University
• AR
  Agricultural Research Service

  Award: 2019-67019-29361
• GL
  Great Lakes Bioenergy Research Center

  Award: DE-SC0018409
• BA
  Biological and Environmental Research

  Awards: DE-AC02-06CH11357, DE-SC0018409, SC0018409
• AN
  Argonne National Laboratory

  Awards: DE-AC02, 06CH11357, AC02-06CH11357