Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster

High-resolution electron microscopy of nervous systems has enabled the reconstruction of synaptic connectomes. However, we do not know the synaptic sign for each connection (i.e., whether a connection is excitatory or inhibitory), which is implied by the released transmitter. We demonstrate that artificial neural networks can predict transmitter types for presynapses from electron micrographs: a network trained to predict six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) achieves an accuracy of 87% for individual synapses, 94% for neurons, and 91% for known cell types across a D. melanogaster whole brain. We visualize the ultrastructural features used for prediction,…

• NS
  National Science Foundation
• WT
  Wellcome Trust
• BI
  Boehringer Ingelheim Fonds
• NI
  National Institutes of Health
• MR
  Medical Research Council

  Awards: MC-U105188491, U105188491