Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry

Proteomics is the large scale study of protein structure and function from biological systems through protein identification and quantification. "Shotgun proteomics" or "bottom-up proteomics" is the prevailing strategy, in which proteins are hydrolyzed into peptides that are analyzed by mass spectrometry. Proteomics studies can be applied to diverse studies ranging from simple protein identification to studies of proteoforms, protein-protein interactions, protein structural alterations, absolute and relative protein quantification, post-translational modifications, and protein stability. To enable this range of different experiments, there are diverse strategies for proteome analysis. The nuances of how…

• NS
  National Science Foundation

  Awards: IIBR-1920268, MCB-2225057, IOS-2025297
• IC
  Indian Council of Medical Research
• II
  Israel Institute for Biological Research
• TH
  Translational Health Science and Technology Institute
• AN
  Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación

  Award: PICT 2019
• NI
  National Institutes of Health

  Awards: S10OD026936, R00GM147304, R21AG074234, R35GM142502, U19AG023122, R01GM087221, R24GM127667
• NI
  National Institute of Standards and Technology