Bridge RNAs direct programmable recombination of target and donor DNA
Palo Alto Institute · Arc Research Institute · +6 more institutions
Abstract
Abstract Genomic rearrangements, encompassing mutational changes in the genome such as insertions, deletions or inversions, are essential for genetic diversity. These rearrangements are typically orchestrated by enzymes that are involved in fundamental DNA repair processes, such as homologous recombination, or in the transposition of foreign genetic material by viruses and mobile genetic elements 1,2 . Here we report that IS110 insertion sequences, a family of minimal and autonomous mobile genetic elements, express a structured non-coding RNA that binds specifically to their encoded recombinase. This bridge RNA contains two internal loops encoding nucleotide stretches that base-pair with the target DNA and the…
Citation impact
- FWCI
- 26.22
- Percentile
- 100%
- References
- 74
Authors
11- MGMatthew G. DurrantCorresponding
Palo Alto Institute, Arc Research Institute, University of California, Berkeley
- NTNicholas T. Perry
Palo Alto Institute, Arc Research Institute, University of California, Berkeley
- JJJames J. Pai
Palo Alto Institute, Arc Research Institute
- ARAditya R. Jangid
Palo Alto Institute, Arc Research Institute, University of California, Berkeley
- JSJanuka S. Athukoralage
Palo Alto Institute, Arc Research Institute
Topics & keywords
- Genetics
- Biology
- FLP-FRT recombination
- DNA
- Recombinase
- Homologous recombination
- Base pair
- Computational biology