Reformulating lipid nanoparticles for organ-targeted mRNA accumulation and translation

Fully targeted mRNA therapeutics necessitate simultaneous organ-specific accumulation and effective translation. Despite some progress, delivery systems are still unable to fully achieve this. Here, we reformulate lipid nanoparticles (LNPs) through adjustments in lipid material structures and compositions to systematically achieve the pulmonary and hepatic (respectively) targeted mRNA distribution and expression. A combinatorial library of degradable-core based ionizable cationic lipids is designed, following by optimisation of LNP compositions. Contrary to current LNP paradigms, our findings demonstrate that cholesterol and phospholipid are dispensable for LNP functionality. Specifically, cholesterol-removal…

• NN
  National Natural Science Foundation of China

  Award: 22205201
• ZU
  Zhejiang University
• NS
  Natural Science Foundation of Zhejiang Province

  Award: LZ23H180003