Single-cell atlas of the human brain vasculature across development, adulthood and disease
University Health Network · University of Toronto · +27 more institutions
Abstract
. Here we performed single-cell RNA sequencing analysis of 606,380 freshly isolated endothelial cells, perivascular cells and other tissue-derived cells from 117 samples, from 68 human fetuses and adult patients to construct a molecular atlas of the developing fetal, adult control and diseased human brain vasculature. We identify extensive molecular heterogeneity of the vasculature of healthy fetal and adult human brains and across five vascular-dependent central nervous system (CNS) pathologies, including brain tumours and brain vascular malformations. We identify alteration of arteriovenous differentiation and reactivated fetal as well as conserved dysregulated genes and pathways in the diseased vasculature.…
Citation impact
- FWCI
- 29.37
- Percentile
- 100%
- References
- 119
Authors
37- TWThomas WälchliCorresponding
University Health Network, University of Toronto, University of Zurich, Toronto Western Hospital, University Hospital of Zurich, Ontario Brain Institute, Krembil Research Institute
- MGMoheb Ghobrial
University Health Network, University of Toronto, University of Zurich, Toronto Western Hospital, ETH Zurich, University Hospital of Zurich, Krembil Research Institute
- MSMarc Schwab
University Health Network, University of Toronto, University of Zurich, Toronto Western Hospital, University Hospital of Zurich, Krembil Research Institute
- STShigeki Takada
University Health Network, University of Toronto, Kyoto University, Toronto Western Hospital, Krembil Research Institute
- HZHang Zhong
University Health Network, University of Toronto, University of Zurich, Toronto Western Hospital, ETH Zurich, University Hospital of Zurich, Krembil Research Institute
Topics & keywords
- Human brain
- Biology
- Cell type
- Endothelial stem cell
- Central nervous system
- Circumventricular organs
- Neuroscience
- Endothelium
- Good health and well-being