Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells
University of California, San Francisco · Gladstone Institutes · +1 more institution
Abstract
Proximity labeling proteomics (PLP) strategies are powerful approaches to yield snapshots of protein neighborhoods. Here, we describe a multiscale PLP method with adjustable resolution that uses a commercially available photocatalyst, Eosin Y, which upon visible light illumination activates different photo-probes with a range of labeling radii. We applied this platform to profile neighborhoods of the oncogenic epidermal growth factor receptor and orthogonally validated more than 20 neighbors using immunoassays and AlphaFold-Multimer prediction. We further profiled the protein neighborhoods of cell-cell synapses induced by bispecific T cell engagers and chimeric antigen receptor T cells. This integrated…
Citation impact
- FWCI
- 33.04
- Percentile
- 100%
- References
- 76
Authors
9- ZLZhi Lin
University of California, San Francisco
- KSKaitlin Schaefer
University of California, San Francisco
- ILIrene Lui
University of California, San Francisco
- ZYZi Yao
University of California, San Francisco
- AFAndrea Fossati
Gladstone Institutes, University of California, San Francisco, Quantitative BioSciences
Topics & keywords
- Interactome
- Chimeric antigen receptor
- Cell
- Computer science
- Epidermal growth factor receptor
- Computational biology
- Nanotechnology
- Cell biology
- Sustainable cities and communities