Nutrient and moisture limitations reveal keystone metabolites linking rhizosphere metabolomes and microbiomes

Plants release a wealth of metabolites into the rhizosphere that can shape the composition and activity of microbial communities in response to environmental stress. The connection between rhizodeposition and rhizosphere microbiome succession has been suggested, particularly under environmental stress conditions, yet definitive evidence is scarce. In this study, we investigated the relationship between rhizosphere chemistry, microbiome dynamics, and abiotic stress in the bioenergy crop switchgrass grown in a marginal soil under nutrient-limited, moisture-limited, and nitrogen (N)-replete, phosphorus (P)-replete, and NP-replete conditions. We combined 16S rRNA amplicon sequencing and LC-MS/MS-based metabolomics…

• UD
  U.S. Department of Energy

  Awards: -AC02-05CH11231, 05CH11231, SCW1555, AC02-05CH11231, DE-AC02, DE-AC02-05CH11231, DE-SC0014079, AC52-07NA27344, DE-AC02-
• UO
  University of Oklahoma
• NR
  Noble Research Institute
• BA
  Biological and Environmental Research

  Awards: 05CH11231, DE-AC52-07NA27344, DE-AC02-05CH11231, AC02-05CH11231, DE-SC0014079
• LL
  Lawrence Livermore National Laboratory

  Awards: DE-AC52-07NA27344, DE-AC02-05CH11231, AC52-07NA27344
• LB
  Lawrence Berkeley National Laboratory

  Awards: DE-AC02-05CH11231, 05CH11231, AC02-05CH11231, DE-AC52-07NA27344