Rfam 15: RNA families database in 2025

The Rfam database, a widely used repository of non-coding RNA families, has undergone significant updates in release 15.0. This paper introduces major improvements, including the expansion of Rfamseq to 26 106 genomes, a 76% increase, incorporating the latest UniProt reference proteomes and additional viral genomes. Sixty-five RNA families were enhanced using experimentally determined 3D structures, improving the accuracy of consensus secondary structures and annotations. R-scape covariation analysis was used to refine structural predictions in 26 families. Gene Ontology (GO) and Sequence Ontology annotations were comprehensively updated, increasing GO term coverage to 75% of families. The release adds 14 new…

• WT
  Wellcome Trust

  Award: 218302/Z/19/Z
• EB
  European Bioinformatics Institute
• NI
  National Institutes of Health
• DF
  Directorate for Biological Sciences
• BA
  Biotechnology and Biological Sciences Research Council

  Awards: BB/S020268/1, BB/S020462/1, BB/S020462/1
• IR
  Intramural Research Program