Discovery and significance of protein-protein interactions in health and disease
University of Toronto · University of California, San Francisco · +1 more institution
Abstract
The identification of individual protein-protein interactions (PPIs) began more than 40 years ago, using protein affinity chromatography and antibody co-immunoprecipitation. As new technologies emerged, analysis of PPIs increased to a genome-wide scale with the introduction of intracellular tagging methods, affinity purification (AP) followed by mass spectrometry (MS), and co-fractionation MS (CF-MS). Now, combining the resulting catalogs of interactions with complementary methods, including crosslinking MS (XL-MS) and cryogenic electron microscopy (cryo-EM), helps distinguish direct interactions from indirect ones within the same or between different protein complexes. These powerful approaches and the…
Citation impact
- FWCI
- 24.02
- Percentile
- 100%
- References
- 179
Authors
3Topics & keywords
- Biology
- Disease
- Computational biology
- Protein–protein interaction
- Genetics
- Evolutionary biology
- Internal medicine
Funding
- UDU.S. Department of Defense
- FHF. Hoffmann-La Roche
- MTMaze Therapeutics
- NINational Institutes of HealthAwards: U54CA274502, U19AI135972, U54AI170792, 1OT2OD032742, U19AI135990
- DADefense Advanced Research Projects AgencyAwards: -0011-19-2-0020, -0011-20-2-0029
- ARAdvanced Research Projects Agency
- CICanadian Institutes of Health ResearchAward: FDN-154338
- NONIH Office of the DirectorAward: 1OT2OD032742
- NCNational Cancer InstituteAward: U54CA274502
- NINational Institute of Allergy and Infectious DiseasesAwards: U54AI170792, U19AI135990, U19AI135972