UniProt: the Universal Protein Knowledgebase in 2025

The aim of the UniProt Knowledgebase (UniProtKB; https://www.uniprot.org/) is to provide users with a comprehensive, high-quality and freely accessible set of protein sequences annotated with functional information. In this publication, we describe ongoing changes to our production pipeline to limit the sequences available in UniProtKB to high-quality, non-redundant reference proteomes. We continue to manually curate the scientific literature to add the latest functional data and use machine learning techniques. We also encourage community curation to ensure key publications are not missed. We provide an update on the automatic annotation methods used by UniProtKB to predict information for unreviewed entries…

• NS
  National Science Foundation
• EM
  European Molecular Biology Laboratory
• EA
  EMBL Australia
• NI
  National Institutes of Health

  Awards: R35GM141873, U24HG007822, HG002273
• DF
  Directorate for Biological Sciences

  Award: BB/X002179/1
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  Biotechnology and Biological Sciences Research Council

  Awards: BB/T010541/1, BB/T015608/1
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  Staatssekretariat für Bildung, Forschung und Innovation
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  Horizon 2020

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  National Institute on Aging

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• NH
  National Heart, Lung, and Blood Institute

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• NH
  National Human Genome Research Institute

  Awards: HG002273, U24HG007822
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  NIH Office of the Director

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• NE
  National Eye Institute

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• NC
  National Cancer Institute

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  National Institute of General Medical Sciences

  Awards: R35GM141873, U24HG007822
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  National Institute of Allergy and Infectious Diseases

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• NI
  National Institute of Diabetes and Digestive and Kidney Diseases

  Award: U24HG007822