Covalent immobilization: A review from an enzyme perspective

• Covalent immobilization increases enzyme stability at the cost of activity. • Immobilization method selection depends on enzyme, material, and application. • Carbodiimide and Shiff base methods are the most common covalent techniques used. • Immobilization strategies need to be carefully tuned to optimize performance. • Immobilized enzyme orientation and conformational changes require further study. Enzymes are indispensable in biotechnology, serving as biological catalysts in applications across various domains, including biosensors, fine chemicals production, pharmaceuticals, and drug development. In this context, enzyme immobilization is crucial for ensuring their retention on devices while preserving…

• AR
  Australian Research Council

  Awards: DP210103160, LP180100309