Review of Lignocellulosic Biomass Pretreatment Using Physical, Thermal and Chemical Methods for Higher Yields in Bioethanol Production

The increasing demand for renewable energy sources has led to significant interest in second-generation biofuels derived from lignocellulosic biomass and waste materials. This review underscores the pivotal role of lignocellulosic biomass valorization in meeting global energy needs, mitigating greenhouse gas emissions, and fostering a circular bioeconomy. Key pretreatment methods—including steam explosion, pressure treatment, and chemical pretreatment—are analyzed for their ability to enhance the accessibility of cellulose and hemicellulose in enzymatic saccharification. Advances in cellulolytic enzyme development and fermentation strategies, such as the use of genetically engineered microorganisms capable of…