A pan-immune panorama of bacterial pneumonia revealed by a large-scale single-cell transcriptome atlas

Abstract Bacterial pneumonia is a significant public health burden, contributing to substantial morbidity, mortality, and healthcare costs. Current therapeutic strategies beyond antibiotics and adjuvant therapies are limited, highlighting the need for a deeper understanding of the disease pathogenesis. Here, we employed single-cell RNA sequencing of 444,146 bronchoalveolar lavage fluid cells (BALFs) from a large cohort of 74 individuals, including 58 patients with mild ( n = 22) and severe ( n = 36) diseases as well as 16 healthy donors. Enzyme‐linked immunosorbent and histological assays were applied for validation within this cohort. The heterogeneity of immune responses in bacterial pneumonia was observed,…

• NS
  National Science Foundation

  Award: 82100096
• NK
  National Key Research and Development Program of China

  Awards: 2021YFC2301102, 2021YFC2301101