Pretargeted Multimodal Tumor Imaging by Enzymatic Self-Immobilization Labeling and Bioorthogonal Reaction
Nanjing University · East China University of Science and Technology · +1 more institution
Abstract
Covalent modification of cell membranes has shown promise for tumor imaging and therapy. However, existing membrane labeling techniques face challenges such as slow kinetics and poor selectivity for cancer cells, leading to off-target effects and suboptimal in vivo efficacy. Here, we present an enzyme-triggered self-immobilization labeling strategy, termed E-SIM, which enables rapid and selective labeling of tumor cell membranes with bioorthogonal trans-cycloctene (TCO) handles in vivo. E-SIM utilizes P-TCO, an alkaline phosphatase (ALP) responsive quinone methide (QM) precursor with a TCO group, facilitating the rapid conjugation of high-density TCO handles onto tumor cell membranes via proximity labeling.…
Citation impact
- FWCI
- 30.96
- Percentile
- 100%
- References
- 60
Authors
17Topics & keywords
- Chemistry
- Bioorthogonal chemistry
- Combinatorial chemistry
- Enzyme
- Biochemistry
- Organic chemistry
- Click chemistry