CRISPR-Based Homogeneous Electrochemical Strategy for Near-Zero Background Detection of Breast Cancer Extracellular Vesicles via Fluidity-Enhanced Magnetic Capture Nanoprobe
Qingdao Agricultural University · Pharmaceutical Biotechnology (Czechia) · +2 more institutions
Abstract
Precise identification and analysis of multiple protein biomarkers on the surface of breast cancer cell-derived extracellular vesicles (BC-EVs) are of great significance for noninvasive diagnosis of the breast cancer subtypes, but it remains a major challenge owing to their high heterogeneity and low abundance. Herein, we established a CRISPR-based homogeneous electrochemical strategy for near-zero background and ultrasensitive detection of BC-EVs. To realize the high-performance capture and isolation of BC-EVs, fluidity-enhanced magnetic nanoprobes were facilely prepared. After capturing BC-EVs, the AND logic gate-based catalytic hairpin assembly (CHA) and the trans-cleavage activity of CRISPR-Cas12a against…
Citation impact
- FWCI
- 30.84
- Percentile
- 100%
- References
- 43
Authors
7- LYLimin YangCorresponding
Qingdao Agricultural University, Pharmaceutical Biotechnology (Czechia)
- JZJingang Zhang
Qingdao University, Qingdao Agricultural University, Affiliated Hospital of Qingdao University, Pharmaceutical Biotechnology (Czechia)
- JZJing Zhang
Qingdao University, Qingdao Agricultural University, Affiliated Hospital of Qingdao University, Pharmaceutical Biotechnology (Czechia)
- THTing Hou
Qingdao Agricultural University, Pharmaceutical Biotechnology (Czechia)
- QGQian Gao
Qingdao Agricultural University, Pharmaceutical Biotechnology (Czechia)
Topics & keywords
- Chemistry
- Nanoprobe
- Homogeneous
- Extracellular vesicles
- Breast cancer
- Biophysics
- Nanotechnology
- Nanoparticle