Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection
University of Science and Technology of China · CAS Key Laboratory of Urban Pollutant Conversion · +2 more institutions
Abstract
The CRISPR-based detection methods have been widely applied, yet they remain limited by the non-universal nature of one-pot diagnostic approaches. Here, we report a universal one-pot fluorescent method for the detection of epidemic pathogens, delivering results within 15-20 min. This method uses heparin sodium to precisely tunes the cis-cleavage capability of Cas12 via interference with the Cas12a-crRNA binding process, thereby generating significant fluorescence due to the accumulation of isothermal amplification products. Additionally, this universal assay accommodates both classic and suboptimal PAMs, as well as various Cas12a subtypes such as LbCas12a, AsCas12a, and AapCas12b. Such a robust method…
Citation impact
- FWCI
- 46.83
- Percentile
- 100%
- References
- 68
Authors
9- ZCZhou‐Hua ChengCorresponding
University of Science and Technology of China, CAS Key Laboratory of Urban Pollutant Conversion
- XLXiyan Luo
University of Science and Technology of China, CAS Key Laboratory of Urban Pollutant Conversion
- SLSteven L. Yu
University of Science and Technology of China, CAS Key Laboratory of Urban Pollutant Conversion
- DMDi Min
University of Science and Technology of China, CAS Key Laboratory of Urban Pollutant Conversion
- SZShuxia Zhang
Fujian Medical University, Union Hospital
Topics & keywords
- Nucleic acid
- Nucleic acid detection
- Chemistry
- Computer science
- Computational biology
- Biochemistry
- Biology
- Zero hunger