Carbohydrate-active enzymes from Akkermansia muciniphila break down mucin O-glycans to completion
University of Birmingham · Technical University of Denmark · +2 more institutions
Abstract
Akkermansia muciniphila is a human microbial symbiont residing in the mucosal layer of the large intestine. Its main carbon source is the highly heterogeneous mucin glycoprotein, and it uses an array of carbohydrate-active enzymes and sulfatases to access this complex energy source. Here we describe the biochemical characterization of 54 glycoside hydrolases, 11 sulfatases and 1 polysaccharide lyase from A. muciniphila to provide a holistic understanding of their carbohydrate-degrading activities. This was achieved using a variety of liquid chromatography techniques, mass spectrometry, enzyme kinetics and thin-layer chromatography. These results are supported with A. muciniphila growth and whole-cell assays.…
Citation impact
- FWCI
- 44.12
- Percentile
- 100%
- References
- 70
Authors
12Topics & keywords
- Akkermansia muciniphila
- Mucin
- Glycan
- Biochemistry
- Glycoside hydrolase
- Chemistry
- Enzyme
- Oligosaccharide