Nature-inspired platform nanotechnology for RNA delivery to myeloid cells and their bone marrow progenitors
Eindhoven University of Technology · Radboud University Nijmegen · +10 more institutions
Abstract
Nucleic acid therapeutics are used for silencing, expressing or editing genes in vivo. However, their systemic stability and targeted delivery to bone marrow resident cells remains a challenge. In this study we present a nanotechnology platform based on natural lipoproteins, designed for delivering small interfering RNA (siRNA), antisense oligonucleotides and messenger RNA to myeloid cells and haematopoietic stem and progenitor cells in the bone marrow. We developed a prototype apolipoprotein nanoparticle (aNP) that stably incorporates siRNA into its core. We then created a comprehensive library of aNP formulations and extensively characterized their physicochemical properties and in vitro performance. From…
Citation impact
- FWCI
- 26.50
- Percentile
- 100%
- References
- 55
Authors
34- SRStijn R. J. HofstraatCorresponding
Eindhoven University of Technology
- TATom Anbergen
Radboud University Nijmegen, Radboud University Medical Center
- RZRobby Zwolsman
Eindhoven University of Technology
- JDJeroen Deckers
Radboud University Nijmegen, Radboud University Medical Center
- YVYuri van Elsas
Radboud University Nijmegen, Radboud University Medical Center
Topics & keywords
- Gene silencing
- Bone marrow
- Small interfering RNA
- Progenitor cell
- RNA interference
- Cell biology
- Biology
- Myeloid