The tetracycline resistome is shaped by selection for specific resistance mechanisms by each antibiotic generation
Washington University in St. Louis · National Institutes of Health · +1 more institution
Abstract
The history of clinical resistance to tetracycline antibiotics is characterized by cycles whereby the deployment of a new generation of drug molecules is quickly followed by the discovery of a new mechanism of resistance. This suggests mechanism-specific selection by each tetracycline generation; however, the evolutionary dynamics of this remain unclear. Here, we evaluate 24 recombinant Escherichia coli strains expressing tetracycline resistance genes from each mechanism (efflux pumps, ribosomal protection proteins, and enzymatic inactivation) in the context of each tetracycline generation. We employ a high-throughput barcode sequencing protocol that can discriminate between strains in mixed culture and…
Citation impact
- FWCI
- 21.56
- Percentile
- 100%
- References
- 102
Authors
7Topics & keywords
- Resistome
- Tetracycline
- Antibiotic resistance
- Antibiotics
- Selection (genetic algorithm)
- Microbiology
- Biology
- Computational biology
- Reduced inequalities
Funding
- NINational Institutes of HealthAwards: 2U01AI123394, T32DK077653
- NINational Institute of Allergy and Infectious DiseasesAwards: T32DK077653, 2U01AI123394
- NINational Institute of Diabetes and Digestive and Kidney DiseasesAwards: T32DK077653, T32-DK007130, DK007130
- NDNHLBI Division of Intramural Research