Individualized patient tumor organoids faithfully preserve human brain tumor ecosystems and predict patient response to therapy

Tumor organoids are important tools for cancer research, but current models have drawbacks that limit their applications for predicting response to therapy. Here, we developed a fast, efficient, and complex culture system (IPTO, individualized patient tumor organoid) that accurately recapitulates the cellular and molecular pathology of human brain tumors. Patient-derived tumor explants were cultured in induced pluripotent stem cell (iPSC)-derived cerebral organoids, thus enabling culture of a wide range of human tumors in the central nervous system (CNS), including adult, pediatric, and metastatic brain cancers. Histopathological, genomic, epigenomic, and single-cell RNA sequencing (scRNA-seq) analyses…

• C
  Carl-Zeiss-Stiftung
• DF
  Deutsche Forschungsgemeinschaft

  Award: 404521405-CRC1389
• UH
  Universität Heidelberg
• DK
  Deutschen Konsortium für Translationale Krebsforschung

  Awards: CRC1389, 404521405-CRC1389 UNITE Glioblastoma B01
• ER
  European Research Council

  Award: 647055