reviewLight Science & ApplicationsFeb 11, 2025GOLD OA

In vivo surface-enhanced Raman scattering techniques: nanoprobes, instrumentation, and applications

Kangwon National University · Harvard University · +3 more institutions

PubMed
Indexed incrossrefdoajpubmed

Abstract

Surface-enhanced Raman scattering (SERS) has emerged as a powerful tool in various biomedical applications, including in vivo imaging, diagnostics, and therapy, largely due to the development of near-infrared (NIR) active SERS substrates. This review provides a comprehensive overview of SERS-based applications in vivo, focusing on key aspects such as the design considerations for SERS nanoprobes and advancements in instrumentation. Topics covered include the development of NIR SERS substrates, Raman label compounds (RLCs), protective coatings, and the conjugation of bioligands for targeted imaging and therapy. The review also discusses microscope-based configurations such as scanning, widefield imaging, and…

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