Design strategies for tetrazine fluorogenic probes for bioorthogonal imaging

Tetrazine fluorogenic probes play a critical role in bioorthogonal chemistry, selectively activating fluorescence upon reaction to enhance precision in imaging and sensing within complex biological environments. Recent structural innovations-such as varied fluorophore choices, spacer optimization, and direct tetrazine integration within a fluorophore's π-conjugated system-have expanded their spectral range from visible to NIR, enhancing adaptability across various applications. This review examines advancements in the rational design and synthesis of these probes. We examine key fluorogenic mechanisms, such as energy transfer, internal conversion, and electron/charge transfer, that significantly influence…

• MO
  Ministry of Education - Singapore

  Award: MOE-T2EP10222-0001
• NN
  National Natural Science Foundation of China

  Awards: 22377083, 22177081, 22277083
• SU
  Singapore University of Technology and Design

  Award: SKI 2021_04_09
• WC
  West China Hospital, Sichuan University

  Award: ZYYC23003