articleJournal of the American Chemical SocietyFeb 13, 2025Closed access

Dual-Locked Fluorescence Probe for Monitoring the Dynamic Transition of Pulmonary Macrophages

Nanyang Technological University · Université Bourgogne Franche-Comté

PubMed
Indexed incrossrefpubmed

Abstract

Pulmonary macrophages undergo dynamic changes in population, proportion, and polarization during respiratory diseases. Monitoring these changes is critical for understanding their roles in pathology, improving the diagnosis, and guiding drug development. However, current analytic methods based on tissue biopsy are invasive and static, limiting their ability to provide such dynamic information. Herein, we report a dual-locked macrophage-specific renal-clearable probe (DMRPNOCas) for the dynamic monitoring of pulmonary macrophages during influenza A virus (IAV) infection. DMRPNOCas activates fluorescence in the presence of two biomarkers (caspase-1 and NO) only coexpressed by M1 macrophages. To optimize the NO…

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