Guide to the structural characterization of protein aggregates and amyloid fibrils by CD spectroscopy

Protein aggregation and amyloid formation are linked to numerous degenerative diseases, such as Alzheimer's or Parkinson's disease. Additionally, protein aggregation plays a crucial role in various biological processes, such as storage of molecules or cell signaling. Protein molecules can form a wide range of aggregates, from oligomers of different sizes to non-specific aggregates and highly ordered cross-β structured amyloid fibrils with diverse morphologies. Circular dichroism (CD) spectroscopy is a widely used technique to study protein structures providing detailed information at the secondary structure level, and is ideal to distinguish and characterize protein aggregates. Despite its potential, CD…

• SF
  Society for Research in Child Development
• GR
  Gedeon Richter
• MT
  Magyar Tudományos Akadémia

  Awards: NAP3.0, NAP2022-I-3/2022
• EL
  Eötvös Loránd Tudományegyetem
• NK
  Nemzeti Kutatási Fejlesztési és Innovációs Hivatal
• RG
  Richter Gedeon Talentum Alapítvány
• NK
  Nemzeti Kutatási, Fejlesztési és Innovaciós Alap

  Awards: PD135510, K138937, ÚNKP-23-3
• NR
  National Research, Development and Innovation Office