Fluorescent Fingerprint Identification of Protein Structural Changes and Disease-Specific Amyloid Beta Aggregates Based on a Single-Nanozyme Sensor Array

The misfolding of amyloid β (Aβ) peptides into an aggregation state is a central hallmark of the onset of Alzheimer's disease (AD). However, conventional methods are mainly focused on detecting a specific Aβ peptide, which makes it difficult to recognize multiple analytes with different topological features and unfolded states at the same time. Here, we propose a simple and universal sensing strategy to construct a fluorescence sensor array by using a single-nanozyme probe combined with three fluorescent substrates as three recognition units to probe the protein structural changes and identify between multiple Aβ assemblies. In this sensor system, the fingerprint-like patterns are produced from the nonspecific…

• NN
  National Natural Science Foundation of China

  Awards: 82071105, 81974317
• SA
  Science and Technology Commission of Shanghai Municipality

  Award: 21XD1421600
• PF
  Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning

  Award: TP2020069