An electrochemical quinine detection approach based on small molecule promoted split aptamer click ligation reaction

Quinine is a natural antimalarial drug that also serves as a food additive; however, excessive consumption can result in harmful side effects. Here, we propose a simple and cost-effective electrochemical strategy for quinine detection based on the small molecule-templated split aptamer click ligation (SMT-SpA-CLR), where the integrity of the aptamer is restored through the template-promoted proximity effect. We first investigate the effects of two splitting sites on the affinity of quinine aptamer and find that the loop of the stem with more Watson-Crick base pairs is more suitable for splitting, which can maintain the affinity to a great extent. The affinity of the Split 1 design ( K d =48 μM) is slightly…

• NN
  National Natural Science Foundation of China

  Award: 21305025
• HI
  Harbin Institute of Technology

  Award: ES202211
• SK
  State Key Laboratory of Urban Water Resource and Environment
• FR
  Fundamental Research Funds for the Central Universities

  Award: HIT.OCEF.2021025