reviewLab on a ChipJan 1, 2025HYBRID OA

A comprehensive review of competitive lateral flow assays over the past decade

JPJulia Pedreira-RincónLRLourdes RivasJCJoan ComengeVSVasso SkouridouDCDaniel Camprubí

Departament de Salut · Barcelona Institute for Global Health · +8 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Competitive lateral flow assays (LFAs) provide a versatile and cost-effective platform for detecting a wide range of molecular targets across fields such as healthcare, food safety, and environmental monitoring, particularly for small analytes or single epitopes that lack suitable bioreceptor pairs. However, the interpretation of competitive LFAs can be challenging due to their counterintuitive output, where the absence of a test line signifies the presence of the target. In this review, we present a comprehensive overview of the fundamental strategies underlying competitive LFAs, explore the mathematical models that quantify assay performance, and outline the critical parameters involved in their design and…

Citation impact

49
total citations
FWCI
25.63
Percentile
100%
References
183
Citations per year

Authors

9

Topics & keywords

Topics
Keywords
  • Flow (mathematics)
  • Risk analysis (engineering)
  • Biochemical engineering
  • Nanotechnology
  • Business
  • Computer science
  • Engineering
  • Materials science
UN Sustainable Development Goals
  • Zero hunger
No related works found for this paper.

Funding