Rules of engagement for condensins and cohesins guide mitotic chromosome formation
University of Edinburgh · University of Massachusetts Chan Medical School · +6 more institutions
Abstract
We used Hi-C, imaging, proteomics, and polymer modeling to define rules of engagement for SMC (structural maintenance of chromosomes) complexes as cells refold interphase chromatin into rod-shaped mitotic chromosomes. First, condensin disassembles interphase chromatin loop organization by evicting or displacing extrusive cohesin. Second, condensin bypasses cohesive cohesins, thereby maintaining sister chromatid cohesion as sisters separate. Studies of mitotic chromosomes formed by cohesin, condensin II, and condensin I alone or in combination lead to refined models of mitotic chromosome conformation. In these models, loops are consecutive and not overlapping, implying that condensins stall upon encountering…
Citation impact
- FWCI
- 28.70
- Percentile
- 100%
- References
- 114
Authors
18- KSKumiko SamejimaCorresponding
University of Edinburgh
- JHJohan H. GibcusCorresponding
University of Massachusetts Chan Medical School
- SASameer AbrahamCorresponding
Massachusetts Institute of Technology
- FCFernanda Cisneros-SoberanisCorresponding
University of Edinburgh
- ISItaru SamejimaCorresponding
University of Edinburgh
Topics & keywords
- Condensin
- Cohesin
- Establishment of sister chromatid cohesion
- Mitosis
- Chromatid
- Chromatin
- Interphase
- Sister chromatids