Hydrogels with programmed spatiotemporal mechanical cues for stem cell-assisted bone regeneration

Hydrogels are extensively utilized in stem cell-based tissue regeneration, providing a supportive environment that facilitates cell survival, differentiation, and integration with surrounding tissues. However, designing hydrogels for regenerating hard tissues like bone presents significant challenges. Here, we introduce macroporous hydrogels with spatiotemporally programmed mechanical properties for stem cell-driven bone regeneration. Using liquid-liquid phase separation and interfacial supramolecular self-assembly of protein fibres, the macroporous structure of hydrogels provide ample space to prevent contact inhibition during proliferation. The rigid protein fibre-coated pore shell provides sustained…

• NN
  National Natural Science Foundation of China

  Award: T2322010 and T2225016
• NS
  Natural Science Foundation of Jiangsu Province

  Award: BK20220120