Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis
University of California, Los Angeles · Howard Hughes Medical Institute · +9 more institutions
Abstract
Abstract Genome editing is transforming plant biology by enabling precise DNA modifications. However, delivery of editing systems into plants remains challenging, often requiring slow, genotype-specific methods such as tissue culture or transformation 1 . Plant viruses, which naturally infect and spread to most tissues, present a promising delivery system for editing reagents. However, many viruses have limited cargo capacities, restricting their ability to carry large CRISPR-Cas systems. Here we engineered tobacco rattle virus (TRV) to carry the compact RNA-guided TnpB enzyme ISYmu1 and its guide RNA. This innovation allowed transgene-free editing of Arabidopsis thaliana in a single step, with edits inherited…
Citation impact
- FWCI
- 43.75
- Percentile
- 100%
- References
- 42
Authors
18- TWTrevor WeissCorresponding
University of California, Los Angeles
- MKMaris Kamalu
University of California, Los Angeles
- HSHonglue Shi
Howard Hughes Medical Institute, Innovative Genomics Institute, University of California, Berkeley
- ZLZheng Li
University of California, Los Angeles
- JAJasmine Amerasekera
University of California, Los Angeles
Topics & keywords
- Genome editing
- CRISPR
- Transgene
- Biology
- Genome
- Computational biology
- Arabidopsis
- RNA
Funding
- NSNational Science FoundationAwards: DE-AC02-05CH11231, 2334027
- HHHoward Hughes Medical InstituteAward: DE-AC02-05CH11231
- UDU.S. Department of EnergyAwards: -AC02-05CH11231, 05CH11231, AC02-05CH11231, DE-AC02, DE-AC02-05CH11231, DE-AC02-
- OOOffice of ScienceAwards: AC02-05CH11231, -AC02-05CH11231, DE-AC02
- BABiological and Environmental ResearchAwards: 05CH11231, DE-AC02-05CH11231, AC02-05CH11231
- LBLawrence Berkeley National LaboratoryAwards: DE-AC02-05CH11231, 05CH11231, AC02-05CH11231