Fe3+ Reducing Power as the Most Common Assay for Understanding the Biological Functions of Antioxidants

Antioxidants counteract the harmful effects of free radicals on metabolism and prevent fatty food degradation during processing and storage. The Fe3+-reducing assay, based on reduction of ferric ions (Fe3+) to ferrous ions (Fe2+) in the presence of antioxidants acting as reducing agents, is widely recognized and used to evaluate the antioxidant capacity of various biological samples, including plant extracts, food, beverages, and pharmaceuticals. Reduction of Fe3+ to Fe2+ is also crucial in biogeochemical cycling, microbial metabolism, and industrial applications. This review comprehensively describes the Fe3+-reducing assay, its adaptation to different analytes, identification of the most potent antioxidants,…

• KS
  King Saud University
• TB
  Türkiye Bilimler Akademisi