Liposomal lipid nanoparticles for extrahepatic delivery of mRNA
University of British Columbia · Canada's Michael Smith Genome Sciences Centre · +3 more institutions
Abstract
Long-circulating, transfection-competent lipid nanoparticle (LNP)-mRNA delivery systems are critical for achieving efficient transfection in extrahepatic tissues. Here we investigate the properties of LNP mRNA systems containing high proportions of bilayer forming lipids, using equimolar egg sphingomyelin and cholesterol as the bilayer-forming components. We show that LNP mRNA systems prepared at bilayer lipid to ionizable lipid molar ratios of 4-0.67 exhibit high mRNA encapsulation efficiencies (90–100%) and excellent transfection potencies in vitro. Systems with bilayer lipid to ionizable lipid molar ratios equating to 4 exhibit a liposomal morphology with a solid core suspended in an aqueous interior…
Citation impact
- FWCI
- 27.06
- Percentile
- 100%
- References
- 37
Authors
17- MHMiffy H. Y. ChengCorresponding
University of British Columbia
- YZYao Zhang
University of British Columbia, Canada's Michael Smith Genome Sciences Centre
- KFKevin Fox
University of British Columbia
- JLJerry Leung
University of British Columbia, Canada's Michael Smith Genome Sciences Centre
- CSColton Strong
University of British Columbia, Canada's Michael Smith Genome Sciences Centre
Topics & keywords
- Liposome
- Nanoparticle
- Computational biology
- Nanotechnology
- Materials science
- Biology