Technical Evaluation of Plasma Proteomics Technologies
Morgridge Institute for Research · Utrecht University
Abstract
Plasma proteomics technologies are rapidly evolving and of critical importance to the field of biomedical research. Here, we report a technical evaluation of six notable plasma proteomics technologies─unenriched (Neat), acid depletion, PreOmics ENRICHplus, Mag-Net, Seer Proteograph XT, and Olink Explore HT. The methods were compared on proteomic depth, reproducibility, linearity, tolerance to lipid interference, and limit of detection/quantification. In total, we performed 618 LC-MS/MS experiments and 93 Olink Explore HT assays. The Seer method achieved the greatest proteomic depth (∼4500 proteins detected), while Olink detected ∼2600 proteins. Other MS-based methods ranged from ∼500-2200 proteins detected. In…
Citation impact
- FWCI
- 24.81
- Percentile
- 100%
- References
- 45
Authors
6Topics & keywords
- Proteomics
- Computational biology
- Computer science
- Chemistry
- Data science
- Biology
- Biochemistry