articleNature CommunicationsMay 16, 2025GOLD OA

Dynamic single-cell metabolomics reveals cell-cell interaction between tumor cells and macrophages

Peking University · Beijing National Laboratory for Molecular Sciences

PubMed
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Abstract

Single-cell metabolomics reveals cell heterogeneity and elucidates intracellular molecular mechanisms. However, general concentration measurement of metabolites can only provide a static delineation of metabolomics, lacking the metabolic activity information of biological pathways. Herein, we develop a universal system for dynamic metabolomics by stable isotope tracing at the single-cell level. This system comprises a high-throughput single-cell data acquisition platform and an untargeted isotope tracing data processing platform, providing an integrated workflow for dynamic metabolomics of single cells. This system enables the global activity profiling and flow analysis of interlaced metabolic networks at the…

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