articleAnalytical ChemistryMay 16, 2025Closed access

A Bimetallic Nanozyme Synergistic Effect-Driven Enzyme Cascade Nanoreactor for Instant Immunoassay

Key Laboratory of Guangdong Province · Fuzhou University

PubMed
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Abstract

This study presents a colorimetric sensor for cancer screening utilizing the bifunctional enzyme activity of NiCo Prussian blue analogue (PBA), a PBA material. By introducing oxygen vacancies and employing a dual-metal doping strategy, NiCo PBA overcomes the limitations in catalytic activity observed in single-metal-doped materials (such as Ni PBA and Co PBA), significantly enhancing both peroxidase-like (POD) and catalase-like (CAT) activities. Compared to single-metal-doped Ni PBA and Co PBA, NiCo PBA exhibited a 30.08-fold increase in POD activity and a 4.83-fold increase in CAT activity, demonstrating higher sensitivity in carcinoembryonic antigen (CEA) detection. By integrating NiCo PBA with a cascade…

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