A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types

The mammalian cortex is comprised of cells classified into types according to shared properties. Defining the contribution of each cell type to the processes guided by the cortex is essential for understanding its function in health and disease. We use transcriptomic and epigenomic cortical cell-type taxonomies from mouse and human to define marker genes and putative enhancers and create a large toolkit of transgenic lines and enhancer adeno-associated viruses (AAVs) for selective targeting of cortical cell populations. We report creation and evaluation of fifteen transgenic driver lines, two reporter lines, and >1,000 different enhancer AAV vectors covering most subclasses of cortical cells. The tools…

• UO
  University of Washington

  Award: SCR_017863
• AI
  Allen Institute
• NI
  National Institute of Mental Health and Neurosciences

  Awards: RF1MH121274, RF1MH114126, UG3MH120095, U19MH114830
• NI
  National Institutes of Health

  Awards: UG3MH120095, RF1MH114126, U19MH114830, RF1MH121274